Automated Next-Generation Sequencing Sample Preparation Methods
We partner with customers and leading technology vendors to develop and deliver the best solutions. Our vast portfolio of Biomek-automated next-generation sequencing sample prep methods are demonstrated to generate quality data using real-world samples, and includes a growing number of Illumina®-qualified NGS methods.
Review our methods list below and supporting application overview documents to find the kits you’re currently working with in your lab or those you plan to evaluate in the future.
DEMONSTRATED METHOD |
BIOMEK i7
HYBRID |
BIOMEK i7/i5
MULTICHANNEL |
BIOMEK i5
SPAN-8 |
BIOMEK FXP
HYBRID |
BIOMEK FXP/NXP
MULTICHANNEL |
BIOMEX NXP
SPAN-8 |
BIOMEK 4000
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CANCER PANELS | ||||||||
Illumina AmpliSeq Cancer HotSpot Panel v2 |
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Illumina AmpliSeq Cancer HotSpot Panel v3 |
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Illumina AmpliSeq Focus Panel |
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Illumina TruSight Oncology 500 DNA/RNA |
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Illumina TruSight Oncology 500 HT |
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Illumina TruSight Cancer |
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Illumina TruSight Tumor 15 |
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Illumina TruSight Tumor 170 |
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Illumina TruSight Tumor 500 |
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NEB NEBNext Direct Cancer HotSpot Panel |
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TruSight RNA Pan Cancer |
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DNA SEQUENCING | ||||||||
ATAC-Seq |
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Illumina AmpliSeq for Illumina NGS |
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Illumina DNA PCR-Free Prep |
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Illumina ForenSeq |
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Illumina Nextera DNA Flex Library Prep Kit |
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Illumina Nextera XT |
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Illumina TruSeq Custom Amplicon Low Input Kit |
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Illumina TruSeq DNA PCR-Free |
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Illumina TruSeq Nano DNA |
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NEB NEBNext Ultra DNA for Illumina NGS (ChIP-seq and HLA) |
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NEB NEBNext Ultra II DNA Kit for Illumina NGS |
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NEB NEBNext Ultra II FS DNA |
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NuGEN Ovation Ultralow Library System v2 |
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NuGEN Ovation Rapid Library System (PCR-Free) |
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Roche KAPA HyperPrep Library Prep Kit for Illumina NGS |
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Roche KAPA HyperPlus Library Prep Kit for Illumina NGS |
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Swift Biosciences Accel - NGS 2S Plus DNA Library Kit for Illumina NGS |
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Takara SMARTer ThruPLEX DNA-seq |
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Twist Library Preparation Kit (Mechanical & enzymatic fragmentation) |
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HLA TYPING | ||||||||
GenDx NGSgo |
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Illumina TruSight HLA Sequencing Panel v2 |
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Immucor MIA FORA Flex HLA |
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Omixon Holotype HLA X2 |
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RNA SEQUENCING | ||||||||
Illumina AmpliSeq for Illumina NGS |
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Illumina RNA Prep with Enrichment |
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Illumina TruSeq RNA v2 |
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Illumina TruSeq RNA Exome |
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Illumina TruSeq Stranded mRNA |
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Illumina TruSeq Stranded Total RNA |
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Lexogen QuantSeq 3' mRNA -Seq Library Prep Kit |
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NEBNext ARTIC SARS-CoV-2 FS Express |
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NEB NEBNext Small RNA Kit for Illumina NGS |
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NEB NEBNext Ultra II Directional RNA Library Kit for Illumina NGS |
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NuGEN Ovation Universal RNA-Seq |
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NuGEN Trio RNA-Seq |
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NuGEN Universal Plus mRNA-Seq |
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Roche KAPA mRNA HyperPrep Kit |
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Roche KAPA RNA HyperPrep Kit with RiboErase (HMR) |
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SINGLE-CELL SEQ | ||||||||
10X Genomics Single Cell 3’ kit- Library Preparation |
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NEB NEBNext Single Cell/ Low Input RNA Library Prep Kit |
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Takara SmartSeq HT |
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TARGET/EXOME CAPTURE | ||||||||
Agilent HaloPlex Target Enrichment - Ion Torrent |
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Agilent SureSelect XT |
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IDT xGen Hybridization and capture of DNA Libraries |
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Illumina Nextera DNA Exome Kit |
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Illumina Nextera Flex Enrichment |
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Illumina Nextera Rapid Capture |
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Illumina TruSeq Exome |
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NEBNext Direct Genotyping Solution |
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NuGEN Allegro Targeted Genotyping |
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Roche Nimblegen SeqCap EZ for Illumina NGS |
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Twist Fast Hybridzation and Wash |
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Twist Standard Hybridization and Wash |
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Method Key
Biomek Automated Workstation Stations and methods are not intended or validated for use in the diagnosis of disease or other conditions. In some cases, method data was generated on pre-production automated workstations.
The automated methods identified are demonstrated methods only and are not validated by Beckman Coulter. Beckman Coulter makes no warranties of any kind whatsoever express or implied, with respect to this protocol, including but not limited to warranties of fitness for a particular purpose or merchantability or that the protocol is non-infringing. All warranties are expressly disclaimed. Your use of the method is solely at your own risk, without recourse to Beckman Coulter. Not intended or validated for use in the diagnosis of disease or other conditions. The demonstrated methods are available through Beckman Coulter automation field applications team.
© 2021 Beckman Coulter Life Sciences. All rights reserved. Beckman Coulter, the stylized logo, and the Beckman Coulter product and service marks mentioned herein are trademarks or registered trademarks of Beckman Coulter, Inc. in the United States and other countries. All additional trademarks used throughout this document are the property of their respective owners. 21.08.2280 AUTO