RNA Extraction from Cells

RNAdvance Cell v2

Reliably extract RNA from cells without the need for filtration or centrifugation.

  • Consistently delivers a high yield of pure nucleic acids
  • Produces high-integrity RNA for NGS and other PCR-based applications
  • Suitable for manual or automated processing

Explore From Cells Models

RNAdvance Cells Workflow Steps

Genomics RNAdvance Cell v2 Workflow

Product Features

Overview
RNAdvance Cell V2 isolates total RNA from cultured cells.

High Quality RNA
The RNAdvance Cell V2 isolation kit purifies RNA with high RIN scores enhancing suitability for downstream applications.

Flexible and Scalable
Combining RNAdvance Blood chemistry with our Biomek Workstation offers high throughput and minimal hands-on solutions: two 96-well plates of samples can be purified in approximately 2 hours and 15 minutes.

Product Specifications

Application Uses RNA Isolation, RNA Extraction, RNA Sequencing, Nucleic Acid Sample Prep
Format Liquid
Starting Sample Material Cultured Cells
Automated Available Yes
Item Specifications Referenced A47943

Citations

Hore, T. A., von Meyenn, F., Ravichandran, M., Bachman, M., Ficz, G., Oxley, D., . . . Reik, W. (2016, October 25). Retinol and ascorbate drive erasure of epigenetic memory and enhance reprogramming to naïve pluripotency by complementary mechanisms. PNAS, 113(43), 12202-1207. doi:10.1073/pnas.1608679113

  • Used to extract RNA from ESC and EpiSC cell lines

Mesnage, R., Phedonos, A., Biserni, M., Arno, M., Balu, S., Corton, J. C., . . . Antoniou, M. N. (2017, October). Evaluation of estrogen receptor alpha activation by glyphosate-based herbicide constituents. Food and Chemical Toxicology, 108, 30-42. doi:10.1016/j.fct.2017.07.025

  • Used to extract RNA from MCF-7 cells

Disclaimer: Beckman Coulter makes no warranties of any kind whatsoever express or implied, with respect to this protocol, including but not limited to warranties of fitness for a particular purpose or merchantability or that the protocol is non-infringing. All warranties are expressly disclaimed. Your use of the method is solely at your own risk, without recourse to Beckman Coulter. Not intended or validated for use in the diagnosis of disease or other conditions. This protocol is for demonstration only, and is not validated by Beckman Coulter.

Content and Resources

Simultaneous DNA & RNA Extraction from Cultured Cells without Splitting Lysate We developed a protocol using Beckman Coulter reagents to isolate both DNA and RNA from the same cultured cell sample. The method utilizes a proprietary buffer to selectively bind RNA and DNA
OEM Reagent Kits Flyer: By partnering with us as an OEM supplier to develop your genomic assays, you’re committing to a solution that you and your customers can rely on.
SPRI Methodology Solid Phase Reversible Immobilization (SPRI) technology, uses paramagnetic beads to selectively bind nucleic acids by type and size.
FormaPure Total Quick Guide Checklist instructions for using the FormaPure Total extraction and purification kit.
FormaPure RNA: Extended Protocol for RNA Isolation from FFPE Sample This document provides instructions for using the FormaPure RNA extraction and purification kit.

Technical Documents