Therapeutics, including small molecules (e.g., antibiotics, chemotherapy agents), biologics (e.g., antibodies, hormones), and nucleic acids (e.g., mRNA, siRNA), are prepared using ready-to-use reagents, liquid handlers, automated cell culture workstations, centrifuges, and chromatography systems.
Biomek i-Series Automated Workstation
Echo 650 Series Acoustic Liquid Handlers
LNP formulations incorporating novel ionizable cationic lipids are at the forefront of RNA delivery technologies. Encapsulation involves using a microfluidic mixer to facilitate the controlled nanoprecipitation of lipids and mRNA in an acidic buffer through electrostatic interaction.
Biomek i-Series Automated Workstation
Optima X Series Ultracentrifuges
Echo 650 Series Acoustic Liquid Handlers
Following encapsulation, buffer exchange is performed to facilitate the rearrangement and stabilization of the nanoparticles. Fractionation of LNPs with different loading levels is also critical and can be achieved with centrifugation to ensure optimal transfection potency.
Biomek i-Series Automated Workstation
Optima X Series Ultracentrifuges
Optima MAX Series Benchtop Ultracentrifuges
Characterizing LNP attributes, such as LNP size, encapsulation efficiency, drug purity, heterogeneity, and mRNA/siRNA loading levels, before in vitro or in vivo use is essential for reproducibility and can be achieved with the Optima AUC Analytical Ultracentrifuge, LS 13 320 XR Particle Size Analyzer, and CytoFLEX Flow Cytometers.
Optima X Series Ultracentrifuges
CytoFLEX nano Flow Cytometer
CytoFLEX Platform
LS 13 320 XR Particle Size Analyzer
